At Zerlotti Genetics, we offer advanced Equine ICSI services designed for breeders who demand precision, efficiency, and results. ICSI allows the production of embryos from valuable mares and stallions when traditional breeding or embryo transfer is not an option; or when maximizing genetics is the goal.
Our in-house laboratory, experienced embryology team, and carefully managed recipient program ensure each embryo is handled with the highest level of care from oocyte to pregnancy.
What Is Equine ICSI?
Intracytoplasmic Sperm Injection (ICSI) is an advanced reproductive technique in which a single sperm cell is injected directly into a mature oocyte using specialized micromanipulation equipment.
This process allows embryo production even with:
- Limited, frozen, or compromised semen
- Mares that cannot carry or flush embryos
- High-value genetics where every oocyte matters
Once fertilized, embryos are cultured in the laboratory and either transferred to a recipient mare or cryopreserved for future use.
When Is ICSI the Best Option?
ICSI is an excellent solution for:
- Performance mares still in competition
- Subfertile or aged mares
- Mares with uterine or cervical issues
- Use of frozen, sexed, or limited semen
- Deceased stallions or rare genetics
- Producing multiple embryos from one mare in a season
Our ICSI Process at Zerlotti Genetics
- Oocyte Collection (OPU)Oocytes are collected via ultrasound-guided aspiration, either on-site or in coordination with approved aspiration teams.
- Oocyte MaturationRetrieved oocytes are matured in carefully controlled laboratory conditions to optimize fertilization success.
- ICSI ProcedureA single sperm is selected and injected directly into each mature oocyte using advanced micromanipulation systems.
- Embryo CultureEmbryos are cultured and closely monitored until they reach the appropriate developmental stage.
- Transfer or CryopreservationViable embryos are either transferred into synchronized recipient mares or vitrified for future use.
Why Choose Zerlotti Genetics?
- Dedicated in-house ICSI laboratory
- Highly experienced embryology team
- Careful handling of high-value genetics
- Personalized case evaluation for each mare and stallion
- Managed recipient mare program
- Transparent communication and professional support
We focus on quality over quantity, giving each embryo the attention it deserves.
Recipient Mare Program
Unlike programs that only perform injections and ship embryos to outside facilities, Zerlotti Genetics manages its own recipient herd, ensuring full control of synchronization, embryo transfer, pregnancy confirmation, and continued care.
Recipient mares are selected, monitored, and managed to support healthy pregnancies and optimal foaling outcomes.
A Smart Investment in Elite Genetics
Equine ICSI allows breeders to preserve, multiply, and advance elite bloodlines with efficiency and precision. Whether your goal is futurity prospects, proven performance crosses, or genetic preservation, ICSI opens the door to possibilities not achievable through traditional breeding.
Schedule a Consultation
Every mare and stallion is unique. We invite you to contact Zerlotti Genetics to discuss your breeding goals and determine whether ICSI is the right option for your program.
“ICSI expertise built before the industry caught on.”















